ugg loafers Neisseria meningitidis

river island ugg boots Neisseria meningitidis

Neisseria meningitidis is a type of Gram negative, pathogenic, aerobic bacteria included among the proteobacteria. These organisms are extremely oxidase and catalase positive, are nonmotile or endospore forming and are extremely susceptible to drying. N. meningitidis bacteria are also diplococci, and therefore resemble coffee beans somewhat in their shape [1]

This bacteria has been found to be the causative agent of bacterial meningitis, a disease that has appeared to date back to the 16th century. However, the disease was only first described in 1805 by Swiss physician Gaspard Vieusseaux[2]. Following Vieusseaux, Italian pathologists Ettore Marchiafava and Angelo Celli also described the micrococci in a sample of cerebral spinal fluid (CSF). The genome of 3 of the 13 serotypes have been sequenced. Strain Z2491, belonging to serotype A, was completely sequenced in September of 2001 by the Sanger Institute. The genome of this particular strain was found to be circular, with a nucleotide length of 2,184,406, was found to contain 2,208 genes, 2,049 protein coding genes, 72 structural RNAs, 87 pseudogenes, 1 contig, be 80% coding and have a GC content of 51% [4]

Strain MC58 (serotype B) was sequenced by the TIGR Center in September of 2001. It’s genome was found to be circular, 2,272,360 nucleotides in length, as well as 79% coding and have a GC content of 51%. Furthermore it was found to contain 2,225 genes, 2,063 protein coding genes, 71 structural RNAs, 91 pseudogenes and no contigs [5].

Sequencing of strain FAM18 (serotype C) was completed by the Sanger Institute in January of 2004 as well and it’s genome was also found to be circular. It was 2,194,961 nucleotides in length, had 2,046 genes, 1,917 protein coding genes, 71 structural RNAs, 58 pseudogenes, no contigs, be 81% coding and have a GC content of 51% [6].

Clearly, the information provided by the genomic sequencing of strains Z2491, MC58 and FAM18 of serotypes A, B, and C, respectively, has enabled researches to discover the means by which this bacteria invade and infect their hosts. Furthermore, without this molecular level understanding, vaccination and treatment are not only made possible, but more efficient as well.

Cell structure and metabolism

The organism has prominent antiphagocytic polysaccharide capsules, which can be classified by immunologic methods:

13 known serogroups; A, B, C, H, I, K, L, M, X, Y, Z, 29E and W135; are grouped on the basis of the capsular polysaccharides which envelope them. Human disease is most often due to serogroups A, B, C, Y, and W135; all serogroup polysaccharides but B, are immunogenic in humans.[7]

10 serotypes on the basis of class 2 or 3 OMP antigens

10 subtypes on the basis of class 1 OMP antigens

13 immunotypes on the basis of lipooligosccharide antigens.

The cell surface of N. meningitidis also possesses type IV pili, which are retractile fibers that serve in their attachment to epithelial cells during host colonization and invasion. N. meningitidis also contains an outer membrane integral protein known as OpcA. This protein’s purpose has been linked to cell adhesion of Neisseria meningitidis to epithelial, as well as endothelial cells via binding to vitronectin and proteoglycan cell surface receptors located within the host. The OpcA protein has been found to function independently of pilus based adhesion [8], mentioned above.

As far as the bacteria’s metabolism is concerned, a study conducted by Archibald and Devoe have provided insight into the dependency for N. meningitidis to metabolize iron in order to maintain cellular functioning [9]. Other studies of the bacteria appear to point to the necessity for the obtainment of glucose, pyruvate, or lactate as a sole carbon source as well [10]. However, Neisseria meningitidis impose no detrimental affects on their hosts as long as they remain in the nasopharyngeal tract. N. The genes of the sequenced serogroups have been found to undergo phase variation more so than any other pathogen studied to date. Accordingly, this ability seems to underlie the bacteria’s expression and contributes to their ability to persevere over the hosts immune system response. Among these known strains, serogroups A, B, and C have been found to be those responsible for 90% of meningococcal meningitis and septicemia cases. Specifically, serogroup A has been implicated in meningitis epidemics in developing countries, while serogroups B and C have been implicated in meningitis epidemics in already developed countries [11].

Neisseria meningitidis is only found in human hosts of which 5 15% of the population are carriers. There is a 3 30% normal carrier state lasting days to months that provides the reservoir for infection of susceptible persons. Specifically, the bacterium can be found in the nasopharyngeal tract, in its asymptomatic form. Transformation from it’s initial asymptomatic form into meningitis arises when the bacterium crosses the mucosal barrier via type IV pili, and enters the blood stream. Once in the blood stream they are free to travel to the cerebral spinal fluid or the meninges, tissues that surround the brain and spinal cord. Accordingly, this infection of the meninges results in bacterial meningitis.

Meningitis results in the swelling of the meninges and causes flu like such as high fever, severe headache, and neck stiffness and pain that make touching your chin to your chest difficult. It is also highly contagious, easily passed to individuals via kissing, sexual contact, coughing and sneezing, giving birth and living in crowded conditions such as dormitories. Risk factors for contracting this disease include time of year (most prevalent during late winter/early spring) being male, age, genetics, a weakened immune system due to a condition such as HIV, and living in crowded conditions as for mentioned. If left untreated meningitis could result in meningococcal septicemia and/or death.

As stated earlier serogroups A, B and C of Neisseria meningitidis are the leading causes of bacterial meningitis. Specifically, of these serogroups, MenB is not only the leading cause of the majority of meningitis cases that develop, but is the only one of the three serogroups that there has yet to be a vaccine developed for.

Taking into account that vaccinations for the other strains were attained via polysaccharide vaccines, current researchers have set out to develop and effective vaccine for serogoup B using outer membrane protein and lipopolysaccharide based approaches as well as “reverse vaccinology” in which systematic search of the bacteria’s genome is performed in order to attain the identity of novel proteins. Furthermore, this technique has enabled researchers to improve their ability to develop better vaccines by “increasing the speed of target identification.”

The research performed in this article used genome scale constraint based metabolic models to analyze culture data, obtain a better understanding of cellular metabolism and develop metabolic engineering strategies for serogroup B Neisseria meningitidis. With the use of a genomic database for this particular serogroup, the main primary metabolic pathways were selected. These pathways included Entner Douderoff and pentose phosphate pathways as well as the tricarboylic acid cycle. glucose and lactate), protein composition, and the amount of fatty acids and lipopolysaccharides, among other biochemical and physiological data, were assessed.

Using the genomic database of Neisseria meningitidis serogroup B along with the biochemical and physiological data obtained, a “genome scale flux model for vaccine process development purposes was constructed.”
ugg loafers Neisseria meningitidis